Biological Media Guide

Carolina offers microbiological media for laboratory use, including pre-poured media and melt-and-pour bottles.

The biological media you choose for your lab can depend on factors such as budget, time constraints, and availability of refrigerator and autoclave space. We offer biological media options that address those factors and help you maintain cultures in a microbiology classroom.

  • Pre-poured media—Using pre-poured plates and tubes saves lab prep time. These ready-to-use media packages are fresh, sterility tested, and come in over 20 varieties. They enable you to provide an entire suite of selective, differential media for your class without having to assemble the ingredients yourself.
  • Melt-and-pour bottles—Give your autoclave a break during prep and save money with our melt-and-pour bottles. These pre-sterilized bottles have a long shelf life. Budget friendly, each 125-mL bottle pours five 100-mm plates or 15 to 20 tubes. Over 25 varieties are available. Please contact Customer Service if you don’t see what you need. We can often create custom bottles.1
  • Media supplies and ingredients—We also stock high-quality ingredients for just about any media you require. Recipes for many popular biological media can be found by downloading our free guide, Techniques for Studying Bacteria and Fungi.

Carolina’s Media Options

Media OptionNumber of Plates or TubesPrep TimeAutoclave Required for PreparationShelf Life
Pre-Poured Media
10 plates per package5 minNoRefrigerated, 2 to 3 weeks for most plates
Tubes10 tubes per package5 minNoRefrigerated, up to 6 months
Melt-and-Pour Bottles (125 mL)5 plates or 15 to 20 tubes75 minNoAt room temperature, up to 6 months
Media Supplies and IngredientsVaries120 minYesVaries

Additional Resources

Bacteria and Media Reference Guide

How to Melt and Pour Agar Plates

Bacteria Care Guide

1Minimum 2-week lead time is required for custom orders. Actual lead time may vary.

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